IMED1002 Lecture Notes - Lecture 7: Antimicrobial Resistance, Ribonucleoprotein, Spliceosome
15 Aug 2018
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Lecture #18: manipulating dna ii: 16th april 2018: Have an understanding of restriction enzyme activity and uses. Understand the process nucleic acids hybridisation including southern blotting, northern blotting and in situ hybridisation. Recombinant dna= dna that has originated from 2 or more dna fragments that are not found together in nature. E. g. from different bacteria human gene with viral dna. Biotechnology industry uses these techniques to develop new products like drugs, hormones, enzymes etc. How can: a specific dna segment be isolated from an entire genome. Researchers can create large sample of dna- tricking replication machinery. Within live bacterial cells (in vivo)- donor dna fragments are inserted into specially designed extrachromosomal. Can carry and amplify gene of interest (vectors) Identification of restriction enzymes (re: ds cuts into the dna (at specific sequences, produced naturally by bacteria to protect against invaders e. g. viruses. Many times- but we will concentrate on type ii.
