BIOL 1123 Lecture Notes - Lecture 2: Frederick Griffith, Ribonuclease, Deoxyribonuclease

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When injected, would cause pneumonia in rats and kill them. It had 2 strains: s (smooth), r (rough). The smooth strain had a smooth outer capsule that would not be able to be destroyed by the rats antibodies, and therefore would kill them. He tried and tested different versions of this bacterium into mice: Living s-strain: mice died, and living strain found in blood. Living r-strain: mice lived, and no bacteria found in mice blood. Denatured s-strain: mice lived, and no bacteria found in mice blood. Denatured r-strain: mice lived, and no bacteria found in mice blood. Living r-strain with denatured s-strain: mice died, and contained living s-strain bacteria! The s-strain bacteria transformed the dead r-strain to recreate copies of itself. It transformed another strain of bacteria for its own gain. Pathogenicity: the ability to cause a disease in the host cell. Avery, macleod, mccarty: purification methods to prove that dna is the genetic material.

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