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BCHM 3P01 (21)
Lecture

The Dark Reactions.docx

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Department
Biochemistry
Course
BCHM 3P01
Professor
Nicholas D Vesprini
Semester
Winter

Description
The Dark ReactionsJanuary2414755 AM Inoxygen photosynthesis you generate ATPNADPH has a high reducing power going to use this to fix carbonNeed photosynthesis to make the cofactors for this reactionDoes glycolysis in the dark as wellTo make sugars from CO2Start with the Calvin cycleoLight reactions turned lights energy to ATP and NADPH biosynthetic reducing powerWe will use this reducing power to reduce Carbon in its most oxidized state CO2 to hexose sugarsEven though they are called the dark reactions they are only active in the light Why What happens in the actual dark The Calvin CycleFirst we make phosphoglycerate from Carbon dioxide and riboluse 15 bisphosphate Isotope LabelingCalvin fed algae 14C labelled Carbon dioxideSeen through mass spec or carbon NMRAfter 5 seconds of light stopped photosynthesis found only a 3 C compound 3 Phosphoglycerate 3PGAfter 60s found a complex mixture
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