BIOL 1F25 Lecture Notes - Lecture 8: Putamen, Grafting, 18 Months
20 Nov 2018
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Document Summary
Treatment of parkinson"s disease with tissue grafts: how long do grafts last, alternative therapies. Long-lasting improvement of symptoms correlates with long-lasting increase in 18f-l-dopa uptake. Staining: applied antibody that binds to tyrosine hydroxylase (th): all cells that contain dopamine also contain th, detected antibody with chemicals that make dark stain. Bottom: high magnification: grafted areas of putamen (left): heavy th staining, non-grafted areas of putamen (right): almost th staining. Grafted areas of putamen: th-immuno-reactive staining found in neurons with cell bodies, axons & other processes, electron micrographs (not shown here): th-containing cells made chemical synapses with other neurons suggests that new neurons from the graft restore movement. Methods: fixed tissues (chemical fixative) cut thin sections (including grafted & non-grafted areas) stained sections with antibodies to detect: tyrosine hydroxylase (th) labels dopamine cells, -synuclei, ubiquitin, examined sections on microscope.
