BIOL 2P02 Lecture Notes - Lecture 30: Prokaryotic Small Ribosomal Subunit, Ribosome-Binding Site, Kozak Consensus Sequence

• School: Brock University
• Department: Biological Sciences
• Course: BIOL 2P02
• Professor: Adonis Skandalis

Document Summary

Midterm review: office hours 11-1130: m and w at 11. Association of mrna + small ribosomal subunit. Ribosome binding site is 8-13 nt upstream of start codon. Sine-dalgarno sequence interacts with ribosomes: puts mrna register between rna of ribosome and mrna, coli initiation. Initiation factor 3 (if3) binds to the 30s ribosomal subunit, freeing it from its complex with the 50s subunit. If1 assists binding of if3 to the 30s ribosomal subunit and blocks the a site of. 30s, helping ensure that the initiation aa-trna fmet-trnafmet can bind only in the p site. If2 gtp binds the initiator fmet-trnafmet and helps it to dock with the small ribosomal subunit. As mrna binds, if3 helps to correctly position the complex such that the trnafmet interacts via base pairing with the rna initiation codon (aug) A region of mrna upstream of the start codon, the shine-dalgarno sequence, (cid:271)ase pairs (cid:449)ith the 3" e(cid:374)d of the (cid:1005)(cid:1010)s rna.