BIOL 2P03 Lecture Notes - Lecture 1: Primary Cell, Flow Cytometry, Differential Centrifugation
27 Feb 2016
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Document Summary
Isolating cells from tissues: isolating organelles from cells, manipulating cellular functions, measuring cellular activities. Microscopy: development of microscopy was essential to development of cell biology. The first light microscopes: beginning in 1600s anton van leeuwenhoek produced a series of compound light microscopes capable of almost 300x magnification. Cell theory: all organisms are composed of cells, the cell is the basic structural and functional unit of organisms, new cells arise from existing cells. Tissues are composed of different types of cells: different cell types have different morphologies and these are related to their different functions. Fluorescence microscopy can see different organelles in either fixed or living cells real time changes in response to stimuli in live cells dynamic nature of different processes: can measure intracellular ca++, ph, membrane potentials, etc. Light microscopy see different cell types and morphological features. Electron microscopy much greater magnification possible; can see organelles and even really big proteins.
