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Oxidative Phosphorylation.docx

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Jeff Stuart

Oxidative PhosphorylationMarch0714801 AM Summary of what we have learned to date What rxns of glucose oxidation generate NADH and FADH2 1GAP13BPG 2Pyruvate Acetyl CoA 3 Isocitrate alpha Ketogluterate 4 vKetogluterate succinyl CoA 5 SuccinateFumerate6 MalateOxaloacetateWhy is rxn 5 in greenThis is the reaction that gives FADH2TestLocation of these reactions Enzymes in the reaction C6H12O66O26CO26H2OG2823 kJmolTransfer of electrons from glucose halfrxnsglucose6water 6 carbon dioxide 24 H24 e6oxygen24 H24 e missed some Energy of glucose oxidation is transferred into the high energy intermediates NADH and FADH2 NADH and FADH2 have a high electron transfer potential ultimately cause reduction of oxygen to water Reaction proceeds indirectly and in doing so allows protons to be pumped across the IMM establishing a proton gradient Why is this important Why not transfer electrons to oxygen directlyoEstablishes the proton motive force for the generation of ATPWithin the ETC there is a progressive handoff of electrons to various intermediates with increasing E valuesRemember E valuesoMeasure of how likely a molecules is going to pick up an electronoE acceptor have a more positive than the E donorsEventually oxygen becomes the E acceptor taking electrons and hydrogen to make water
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