BIOL 3P30 Lecture Notes - Lecture 32: Amphiprioninae, Enzyme Kinetics, Turnover Number
Document Summary
Maintenance of enzyme function in a cold-evolved fish. In the clown fish it has higher activity at its environmental temperature, just like the cod. The enzyme has evolved to work at lower temperature. At 0 degrees that enzymes is more active in the cod o. More cs being made or adjustment made to the enzyme. The number of s p per active site for minute. Number of reactions catalyzed per unit time per enzyme. Kcat ldh in fish species from different environments. Data points are from different species; kcat measurements done at 0 degrees c. More rapid conformational changes in the enzyme evolved to work in the cold. The amino acid changes are plenty so it"s hard to know which ones are. Do these kind of experiments in e coli or yeast. More flexible proteins are more thermally labile (more susceptible to denaturation at higher temps) Same paper as the very first figure for this lecture.