BIOL 3P30 Lecture Notes - Lecture 35: Beta Oxidation, Glycolysis, Acetyl-Coa
22 Aug 2015
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Propose a experiment: x-ray crystallography, nmr, ect, computer program. It is suggested that the end form reacts with fad to modify it: monitor changes to structure of cofactors. Propose an experiment: test through uv spec (like we do in lab) What about unsaturated fatty acids: recall that unsaturated fatty acids have a cis isomerism, enonyl-coa is trans. Hydratase can not bind cis: so we have enonyl coa isomerase, polyunsaturated require further enzymes, some oragnisms are restricted in their fa metabolism (e. g. archae) 7, why: what is the overall reaction, palmitoyl-coa + 7coash +7fad+7nad++7h2o -> 8acetylcoa. 7fadh2 at 1. 5 atps per fadh = 10. 5. 7nadh at 2. 5 atps per nadh = 17. 5. 8 gtps at 1 atp per gtp = 8. 24 nadh at 2. 5 atps per nadh = 60. 8 fadh at 1. 5 atps per fadh = 12. But remember we have to take out 2 atps for activation: so 106 atps per one palmitate!!
