BIOL 3P34 Lecture Notes - Lecture 6: Resting Potential, Lysine, Reference Electrode
10 Oct 2016
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Contour map cupola cdna cloned calf muscle: 2 -like mrnas g e. Inner vestibule doesn"t open as a hole to the cytoplasm. : fetal (and dennervated muscle); long open-time and small conductance. : innervated adult muscle; short open-time and larger conductance. Different subunit composition of channels: different functional properties. Neuronal nachrs: alpha and beta subunits (12 different one) Expression of channels using xenopus oocytes (do not normally express nachrs) Serines in m2 region (hydrophilic) replaced with alanines (weakly hydrophobic) Channels activated by depolarization: voltage-activated na+ channel (ap, voltage-activated k+ channel (ap, voltage-activated ca2+ channel (synaptic transmission) Muscle derived tissue of electric organ delivers electric shocks. 4 subunits (33 -36kda) expressed in mammalian brain: affect channel kinetics and voltage dependence. Each domain has multiple transmembrane helices s1 to s6. *****s5-6**** linker: p loop dips into the pore. S6 region from all 4 domains meet together and line the pore.
