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BIOL 3P50 (28)

Class Notes for BIOL 3P50 at Brock University

Molecular Genetics

Exam Study Guides for BIOL 3P50

Covers all exam topics.

BIOL 3P50 Lecture Notes - Lecture 1: Leucine, Phenylalanine, Peptide

BTEC 3P50 Textbook Notes – Chapter 2 Double Helix • Two DNA chains of the double helix are held together by hydrogen bonds between pairs of bases on opposing strands • Base pairing o Adenine ...

Biological Sciences
BIOL 3P50
Amanda Lepp
BIOL 3P50 Lecture 4: Chapter-4

BTEC 3P50 Textbook Notes Chapter 4 DNA Structure Composed of two polynucleotide chains twisted around each other in the form of a double helix Backbone of each strand of helix is composed of alternating sugar and phosphate...

Biological Sciences
BIOL 3P50
Amanda Lepp
BIOL 3P50 Lecture Notes - Lecture 5: Ribonuclease P, Counterion, Intron

BTEC 3P50 Textbook Notes – Chapter 5 RNA • Backbone contains ribose  -OH at 2’ position • Uracil in place of thymine o Lacks methyl group at 5’ position o Pairs with adenine • Singl...

Biological Sciences
BIOL 3P50
Amanda Lepp
BIOL 3P50 Lecture Notes - Lecture 6: Allosteric Regulation, Glycosylation, Immunoglobulin Light Chain

BTEC 3P50 Textbook Notes – Chapter 6 Basics • Amino acids o α-C is a chiral center o Amino acids that occur in ordinary proteins have the L-configuration o Categorized by properties...

Biological Sciences
BIOL 3P50
Amanda Lepp
BIOL 3P50 Lecture Notes - Lecture 3: Hydrophile, Dipeptide, Cell Nucleus

BTEC 3P50 Textbook Notes – Chapter 3 Characteristics of Chemical Bonds • Strength of bonds o Strong bonds almost never fall apart at physiological temperatures o Weak bonds are easily broken and...

Biological Sciences
BIOL 3P50
Amanda Lepp
BIOL 3P50 Lecture 7: Chapter-7

BTEC 3P50 Textbook Notes Chapter 7 Nucleic Acids Gel electrophoresis separates DNA and RNA molecules according to size when subject to an electric field o DNA is negative, and will migrate through gel towards positive pole...

Biological Sciences
BIOL 3P50
Amanda Lepp
BIOL 3P50 Lecture Notes - Lecture 9: Primase, Okazaki Fragments, Exonuclease

Midterm: 55 all MC Bring clicker. (Review & chapter 7) Chapter 9: February 8, 2017 • In a quadruple time-of-flight tandem mass-spectrometer, peptides of different masses always arrive at the detector at different...

Biological Sciences
BIOL 3P50
Amanda Lepp
BIOL 3P50 Lecture Notes - Lecture 16: Dna Mismatch Repair, Dna Replication, Enol

~ mispairing occurs during replication ~ DNA is very fragile ~ can undergo spontaneous damage ~ hydrolysis leading to loss of base pairs ~ base deamination ~ DAM of C leads to U; not natu...

Biological Sciences
BIOL 3P50
Amanda Lepp
BIOL 3P50 Lecture Notes - Lecture 15: Polyadenylation, Histone H1, Tata Box

~ short region of DNA double helix (2nm) ~ DNA wrapped around histones; forms chromatin (11nm) ~ 30nm fiber packing of nucleosomes ~ extended scaffold-associated chromatin (300nm) ~ seen during interphase ~ conde...

Biological Sciences
BIOL 3P50
Amanda Lepp
BIOL 3P50 Lecture Notes - Lecture 3: Xist (Gene), Exon, Repressor

Biol 3P50 Exam Review Holliday Junction • Step 5: Resolution of Holliday junction •...

Biological Sciences
BIOL 3P50
Alan Castle
BIOL 3P50 Lecture Notes - Lecture 1: Hydrogen Bond, Pancreatic Ribonuclease, Sodium Acetate

1)What is the relationship between the following: a) plasmid size b) plasmid copy number c) plasmid DNA yield Because the genomic DNA is so large, it is broken by shear forces when the cell is lysed, while the plasmid DN...

Biological Sciences
BIOL 3P50
Alan Castle
BIOL 3P50 Lecture Notes - Lecture 18: Reca, Rad51, Archaea

Lecture 17 Homologous recombination at the molecular levelDNA view of strand exchangessDNA within the filament is underwound and stretchedThree nucleotides per RecA, followed by large step before the next baseHomology sea...

Biological Sciences
BIOL 3P50
Mac Neil Lori
BIOL 3P50 Lecture Notes - Lecture 16: Gene Knockout

Lecture 15 Homologous recombination at the molecular levelCrossing overHomologous recombinationBreaking and rejoining of two DNA molecules in regions sharing sequence homology to produce new (recombinant) DNA moleculesImp...

Biological Sciences
BIOL 3P50
Mac Neil Lori
BIOL 3P50 Lecture 17: Homologous recombination at the molecular level 2

Lecture 16 Homologous recombination at the molecular levelHR in E. coliUnlike eukaryotes, E. coli has no protein that introduces DSBsDSBs are generated from DNA damage, mishaps during DNA repair, or collapse of a replicati...

Biological Sciences
BIOL 3P50
Mac Neil Lori
BIOL 3P50 Lecture Notes - Lecture 19: Base Pair

RecQ HelicasesObserved to promote an alternative noncrossover pathway for Holliday Junction processing in humansProcess recombination intermediates to collapse joint molecules before establishment of a double Holiday junct...

Biological Sciences
BIOL 3P50
Mac Neil Lori

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