BIOL 3P50 Lecture Notes - Lecture 16: Dna Mismatch Repair, Dna Replication, Enol

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~ hydrolysis leading to loss of base pairs. ~ dam of c leads to u; not natural in dna. ~ dam of 5-methyl c leads to t; natural in dna. ~ dam of a leads to hypoxanthine; can pair with c. ~ dam of g leads to xanthine, can still pair with c. ~ transitions: purine to purine or pyrimidine to pyrimidine. ~ tautomeriation can occur in all base pairs. ~ enol form of t can pair with g. ~ imino form of c can pair with a. ~ imino form of a can pair with c. ~ replication errors not corrected by proofreading corrected instead by mismatch repair. ~ dam methylase methylates the a in both strands of dna. ~ allows identification of parent or template strand. ~ eukaryotes do not methylate; rely on nicks to identify lagging strand.

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