BIOL 3P51 Lecture Notes - Lecture 8: Massive Parallel Sequencing, Human Genome Project, Dna Sequencing
27 Nov 2018
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Document Summary
11/19/2018: human genome project, sequencing of the human genome was undertaken in 1990, publicly funded billion, 15-year project. Investigate ethical, legal, societal implications: dna sequencing methods, until recently, sanger dideoxy sequencing was the predominant method, sequences a single purified dna fragment. In 1999, private company celera (craig venter) announced it would sequence the genome in 2 years using whole-genome shotgun method. In addition to the human genome, the human genome project also sequenced the genomes of several model organisms: e. coli, s. cerevisiae, c. elegans, drosophila melanogaster. 11/19/2018: protein-coding genes, rna genes, regulatory elements i. e. enhancers, all of the above, a and b only, comparative genomics genome insights, ~ 5% of our genome shows strong conservation with other mammalian genomes. What are some differences: size, no introns, location, dna length. 11/19/2018: linear vs circular, copy number, a snapshot of the nuclear genome, ~7% of our nuclear genome is located in constitutive heterochromatin, remaining 93% is euchromatin.
