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seminar write up 3.docx

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Caroline Starrs

Assignment for Seminar 3 March 3, 2014 Rebecca Lefebvre 5159199 The article, The Systemic Effects of Platelet-Rich Plasma Injection, explores the effects platelet- rich plasma (PRP) has on the human body growth factors and how they can be identified in professional athletes (Dragoo et al p 186). PRP is mostly used for helping athlete’s with tendon, ligament and muscle injuries but, PRP is considered a performance enhancing drug by the World Anti-DopingAgency (WADA) and in banned from all professional competitions due to the insulin growth factors they contain (p 186). The growth factors are argued to give an unfair advantage to athletes and in this study the researches try to determine if this is in fact true (p 187). There were 25 patients involved in the study, 19 men and 6 women, which fit the requirements of not having any health concerns that would interfere with the results of the data such as treatment with hormone therapy and being available for all appointments (p 187). The tests were done according to WADA guidelines and the patients were injected with leukocyte- rich PRP (p 187). Blood samples were collected 0.25,3,24,48,72, and 93 hours after PRP was injected into the patients, and only after they ate and exercised (p 187). The samples were taken from the opposite arm than the injection, processed following strict set instructions from the Leukocyte-rich PRP manufacturer, were stored in polypropylene tubes at -80 degrees Celsius, and were only thawed and retested twice (p 187). There were six growth factors examined in this study which included: human growth hormone (hGH), insulin-like growth factor-1 (IGF-1), insulin- like binding protein-3 (IGFBP-3), basic fibroblast (bFGF), vascular endothelial (VEGF), and platelet- derived-BB growth factors (PDGF-BB) (p 187). hGH, IGF-1, and IGFBP-3 all showed similar results that are seen after regular exercise and the rese
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