SCIE 1P51 Lecture Notes - Lecture 12: Gene Gun, Dna, Gene Delivery
16 Feb 2016
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Science 1p51 lecture twelve february 12th, 2016. Found only in bacteria destroys invading dna; cuts. Not found in humans purified for lab use in test tube. Useful for cutting out a section of the dna containing what you want such as a gene. Two set of ends are sticky and will match: plasmids. Dna from plasmid and gene can be glued back together. New dna containing your gene of interest. Non-viral: vectors must be able to target desired cells, vectors must not induce an immune response. In vivo: vector & gene/plasmid administered directly to organism, and transfers genetic information to organism cells in vivo information to cells ex vivo (or in vitro), then cells are administered to organism. Ex vivo: vector & gene/plasmid used to transfer genetic. Model system: mouse genome common model. Scid severe combined immuno-deficiency: recessive disease, vector, mouse retrovirus. Jesse gelsinger 1999: 18 years old, genetic liver disorder. Research lab looking into the genetic disorder (university of.
