SCIE 1P51 Lecture Notes - Lecture 12: The Techniques, Plasmid, Jesse Gelsinger
16 Sep 2016
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Document Summary
The techniques used to replace a non-functioning or mutated gene or gene sequence, associated with a disease/disorder, with a normal gene to restore function: corrects dna at the genetic level, make deficient protein, can last a lifetime. Found only in bacteria: - destroys invading dna cut dna at specific sites. Not found in humans purified for lab use in test tube. Useful for cutting-out a section of the dna containing what you want such as a gene. Small, circular pieces of dna: ring of dna, few genes. Have a replication signal: can make lots of copies of whatever gene you want. Usually antibiotic resistance: allows the bacteria to survive. Dna from plasmid & gene can be glued back together. New dna containing your gene of interest. Make any protein you want: don"t know all of them. ***put markers on the gene so you can see them. Gene delivery systems: viral, non-viral vectors.
