BIOL 2104 Lecture 1: Prokaryotic Gene Regulation I

• School: Carleton University
• Department: Biology
• Course: BIOL 2104
• Professor: Ingrid L.Stefanovic

Document Summary

04/07/2014: overview: transcriptional regulation, structure of operons, transcription factors (regulatory proteins) and allosteric effectors, overview of lac operon5. Genetic dissection of lac operon: (negative control, catabolite repression of lac operon (positive control) (cid:1) (cid:1) (cid:1) Review of basal prokaryotic transcription (cid:1) (cid:1) (cid:1) (cid:1) (cid:1) (cid:1) (cid:1) (cid:1) (cid:1) Organism must express the enzymes required to survive in that environment. Therefore, want to make enzymes when required. Constitutive: always expressed, housekeeping , e. g. glucose metabolizing enzymes, glucose is the preferred carbon source for bacteria. Adaptive: inducible , made only when needed, e. g. lactose metabolizing enzymes, made only if lactose is the sole carbon source, bacteria, not made if glucose is present. Prokaryotic gene regulation (cid:1) (cid:1) (cid:1) (cid:1) (cid:1) (cid:1) (cid:1) (cid:1) (cid:1: need to be responsive to environment, 2 main control points: Regulatory protein to control transcription of enzymes & structural proteins.