BIOL 2107 Lecture Notes - Lecture 13: Gene Conversion, Spo11, Holliday Junction

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Lecture 13: dna structure, replication, and recombination ii. A. kornberg and others worked out the biochemical aspects of replication in e. coli: they worked with e. coli because it produces a lot of dna, its cheap, accessible and overall efficient to work with. Energy for dna synthesis comes from high-energy phosphate bonds associated with dntps. Dna polymerase (pol) catalyzes new phosphodiester bonds. Highly coordinated process has two stages: initiation proteins open up the double helix and prepare it for complementary base pairing. To reach the sequence of the amino acid, the double helix needs to be opened up and must stay open too: elongation proteins connects the correct sequence of nucleotides on newly formed dna stands. Dna synthesis proceeds in a 5" to 3" direction. Template and newly synthesized strands are antiparallel. Dna polymerase adds nucleotides to 3"-oh of the new strand. Polymerase reads dna from the 3" to 5". Initiation begins at the origin (ori) of replication.

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