BIOL 2107 Lecture Notes - Lecture 4: Rna Splicing, Intron, Haploinsufficiency

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Rna splicing - removes introns from a primary transcript. Exons sequences found in a gene"s dna and mature mrna (expressed regions) Introns sequences found in dna but not in mrna (intervening regions) Rna processing splicing out introns and joins adjacent exons: Short sequences in the primary transcript dictate where splicing occurs. Splicing is catalyzed by the spliceosomes: advantage of alternative splicing. Alternative splicing can produce two different mrnas from the same gene. Occurs in c. elegans and a few other organisms. factors involved in translation. Transfer rnas (trnas) mediate translation of mrna codons to amino acids. Secondary structure (cloverleaf shape) is formed because of short complementary sequences within the trna. Tertiary structure (l shape) is formed by 3-dimensional folding codon and anti-codon sequences aminoacyl-trna synthetase. Aminoacyl-trna synthetases catalyze attachment of amino acids to specific trnas. Wobble: some trnas recognize more than one codon for the amino acid they carry. Consist of several ribosomes translating the same mrna.

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