BIOL 2303 Lecture Notes - Lecture 4: Sulfur, Enzyme, Escherichia Coli

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Metabolism: the chemical reactions and processes required to maintain life. Major case of death in individuals with cystic fibrosis. To grow microbes in lab, they must be supplied with all needed nutrients. Major elements: carbon - 50, oxygen - 17, nitrogen - 13, hydrogen -8. 2, phosphorus - 2. 5, sulfur - 1. 8% Complex media: mixtures of compounds of imprecise composition. Most media can be made as either solid or liquid through the addition of agar. Microbes obtain energy from a variety of sources: organic molecules. Obtain energy from organic molecules (ex: glucose, acetate, ethanol) Organic molecules typically broken down via oxidation. Obtain energy from inorganic molecules (h2, h2s, nh3) Groups of organism specializing on a particular inorganic molecule are known by reference to that molecule. Typically broken down via oxidation = atp. Oxygenic photosynthesis produces oxygen, anoxygenic photosynthesis does not. Regardless of the source of energy, it must be stored chemical in the cell.

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