BIOL 2303 Lecture Notes - Lecture 3: Vitamin K, Streaking, Saccharomyces
21 Mar 2016
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Document Summary
The chemical reactions and processes required to maintain life. To grow microbes in the lab, they must be supplied with all needed nutrients and sterile equipment. Different microbes have different requirements, need these specialized conditions. Anaerobic vs. aerobic bacteria (most bacteria are anaerobic) Need the molecules that keep them alive. Macromolecules: proteins, nucleic acids, lipids, polysaccharides (mostly proteins) Major elements: c, o, n (in amino acids), h, p (phospholipids, dna), s (in some amino acids) Dry weight of cell is mostly carbon and protein. Need sterile media or else there may be other organisms that can grow and take over media. Defined media: precise amounts of pure chemicals, exact composition of these chemicals is known. May only support one or a few organisms which can be a good thing. Complex media: mixtures of compounds of imprecise composition. Ex/ enzymatic digests of casein (milk protein), yeast extract or animal products.
