BIOC 2300 Lecture 4: L4 Nucleic Acids, the Central Dogma

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Niewmann-pick c disease: early in childhood, genetic, in ns there is the highest rate in the world. Dna denaturation and renaturation: double helix is stabilized by h-bonds, mg2+ binding to po4 backbone, and by the hydrophobic effect, tm increases with gc content (due to base stacking, denaturation is reversible: renatuation (tm 20-25 deg c) If you cool it to quickly it will bind incorrectly. Physical view: nucleosome is the basic structural unit of chromatin, condensation is due to basic histone proteins interacting with acidic dna backbone, histone modifications help regulate packing and gene expression (epigenetics, entire mitotic chromosome is 1400 nm. Genetic view: gene is the basic unit of heredity. Initiates transcription at the 5" end of a gene: the promoter region: nucleotide sequence for mrna corresponds to the coding (sense) strand of the original dna and to the codons for translation, mrna synthesis: In prokaryotes, a single transcript often includes several co-regulated genes (an operon), does not contain introns.

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