BIOC 3400 Lecture Notes - Lecture 18: Polyacrylamide Gel Electrophoresis, Chain Termination, Base Pair
11 Nov 2015
School
Department
Course
Professor
Document Summary
Study online at quizlet. com/_1oed6m: chain-terminator sequencing (sanger sequencing, automated. Dna sequencing enzymatic synthesis of polynucleotide chains complementary to template Dna; synthesized chains are terminated at specific nucleotide positions; resolved the fragments on a polyacrylamide gel to obtain the sequence. All four dntps (large amounts), one can be radioactive labeled; One of four ddntps, at very low concentration. [in each well of the gel, it contains fragments that are made from all four dntp and one ddntp, for example, datp, dctp, dttp, dgtp, and ddatp) Chain terminator sequencing needs equal amount of all four dntps and one radioactive laveled ddntp. Pcr-like, only one primer. use fluorescently-labeled ddntps: procedure in automated. Dna sequencing: what are two types of primers used in dna sequencing, shotgun sequencing, shotgun sequencing to obtain genomic sequences. Randomly breaking up dna sequences into lots of small pieces and then reassembling the sequence by looking for regions of overlap.
