BIOC 4403 Lecture Notes - Lecture 10: Rolling Circle Replication, Bacterial Conjugation, Genomic Island
27 Mar 2017
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Bacterial transformation: donor cell undergoes lysis and dies, releases dna and recipient cell takes up the dna. Bacterial transduction: phage-infected donor cell releases the phage, phage injects dna into recipient cell (can be general specialized) Bacterial conjugation: both cells are alive and in contact, complex process involving chromosomal integration of a plasmid. Integrative and conjugative elements (ices): bacterial mobile genetic elements that primarily reside in the host chromosome, but can excise and be transferred to other cells by conjugation. Ices are highly mosaic they contain a variety of different genes associated with conjugation and mobility. Integration and excision are mediated by integrase and excisionase proteins, which mediate dna recombination at att sites in the bacterial chromosome and ice sequences. Conjugative transfer typically occurs via assembly of a type iv secretion system" though which dna passes by rolling circle replication. Many genomic islands are ices that have lost the ability to mediate excision and/or conjugation.
