BIOL 2020 Lecture Notes - Lecture 2: Endoplasmic Reticulum, Sodium Channel, Transmembrane Protein
31 Aug 2016
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The cell ensures that the correct molecules arrives at the correct membrane, or inside the appropriate organelle. Molecular mass daltons or kilodaltons = 1 dalton is 1. 66x10^-27 kg. To figure out the size of a protein measure in moles by using size in kda. Compartments are not independent they form an integrated functional unit. Endomembrane system = a biosynthetic pathway (synthesis, sorting, secreting) endoplasmic reticulum + golgi complex + endosomes + lysosomes + plasma membrane. Protein secretion: lumen proteins synthesis, membrane proteins synthesis. Cell fractionation break down the cell into its simple parts. Microsomes free ribosomes that are not attached to the er. Used to measure the amount of protein that is synthesized at one point in time. Autoradiography used to visualize the pulse chase experiment. Study the secretion pathway of a cell by targeting heat sensitive mutants at specific steps.
