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BIOL 3044 (1)
Lecture

Transgenic Organisms - Section 3

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Department
Biology
Course
BIOL 3044
Professor
billpohajdak
Semester
Winter

Description
08092014000100Lecture112102014FLAVRSAVR1994FDAapprovesthetomato1997approvedbyhealthCanadaItwasthefirstgeneticallymodifiedfoodTakenoffthemarketplacein1998DelayedripeningProlongedshippingshelflifeImprovedqualityIncreasedviscosityPectinsMadeupoflongchainspolysaccharidesofmainlygalacturonicacidGivesplantsfirmnessstructuralsupport35ofcellwallrestiscellulosehemicelluloseandproteinTomatoesarenormallypickedoffthevineearlytheyhavethickskinThesegreentomatoesarelatersprayedwithethylenewhichtriggersripeningThetasteorflavorofthetomatoisbetterifitisleftonthevinebutFLAVRSAVRallowsthetomatotoripenonthevineandstillbefirmforshippingFlavrSavrgeneisantisensetopolygalacturonaseenzymeencodinggeneTheyknewthatnormallywhentheybecomesoftthewallsfallapartbythisenzymePectinisdigestedbythisenzymeThiswasdonemostlyasasellingfeatureAlotofthiswasalreadydonewithtobaccoTomatoesbelongtotobaccofamilybutdontcontainnicotineEverytimeweconsumedoneofthesetomatoeswewereconsumingtoxinSoifitwasntconsumedinmoderationitisveryharmfultohumansTheytestedallthetoxinsproteinslipidsetcandeverythingcamebackthatthesetomatoeswerethesameaseveryothertomatoItwasdiscontinuedbecausewhentheyweredoingthisinCaliforniatheypickedtomatoesthatgrewwellthereTheyarentreallyknownfortomatofarmingandtheyshippedittoFloridasoitdidntgrowwellthereortasteasgoodItalsohadanegativeconsumerresponsefortheretomatopasteCampbellssoupfundedallthisresearchTheGMtomatoesallowedforthickersaucepasteTheymarketeditanditoutsoldtheregularproductInUKtheyhadtheGMOlabelsotheymadethecansbiggerandsoldthemforthesamepriceinthestateswithoutthelabelbuthadlessinitAfterthisthenegativepressanduprisingitwasremovedin1998ThetechnologywassoldandMonsantoownsallthepatentsPurpletomatoEnrichmentoftomatofruitwithhealthpromotinganthocyaninsbyexpressionofselecttranscriptionfactorsTheyarethoughttobecancerfightingWeneedoxygenbutbecauseofitsweirdpropertiesitcanalsobetoxicItformsradicalcompoundsandtheyarethoughttodamageourproteinslipidsetcWhenwegetoldwearedamagedbytheseradicalsThesecompoundsinhibitthoseradicalsAnyfruitorvegetablesthatareverydarkhavethisTheywantedtotaketomatoesandmakethemoverexpressDelandRos1andputthemintoavectorPutthatvectorintothebacteriaandthenputthebacteriaintotheplantThetomatoescameoutdarkdarkpurpleskinandfleshTherewasnodifferenceinthetasteTheywantthegenestobeexpressedinthepollenTheydidthiswithap53knockoutmouseIfthep53isgonethetumordevelopsTheygavemicethreeoptionschowchowtomatoandchowpurpletomatoesPurpletomatoesaddedabout40daystothemicelifespanHigheranthocyaninexpressionmeanshigherantioxidantpropertiesTheyvealsobeentryingtoincreasethelycopeneandithelpsinoxygencaptureCanbeharmfulforsmokersLecture122122014GoldenRiceToletricehavealongshelflifetheyhavetoremovethehuskovertheendospermVitaminAdeficiencyismoreprominentinchildrenItcausesblindnessandimmunesystemissuesMorethan100millionchildrenareaffectedVitaminAistoxicifconsumedincopiousamountsCaroteneisnottoxicWecantakeinalotofitbutweonlybreakdownwhatweneed
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