JOUR 3440 Lecture Notes - Lecture 15: Papaya, Rna Interference, Tumor Suppressor Gene
24 Oct 2015
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Document Summary
Rna interference is a biological process in which rna molecules inhibit gene expression, typically by causing the destruction of specific mrna molecules. Also known as co-suppression, post transcriptional gene silencing (ptgs) 3a silencing gene expression by targeted degradation of mrna using short ds-rnas. 4a a multiprotein complex that incorporates one strand of a small interfering rna (sirna) or microrna (mirna). Risc uses the sirna or mirna as a template for recognizing complementary mrna. When it finds a complementary strand, it activates a protein within risc (argonaute) and cleaves the. 5a: chemically synthesize the si-rna, produce a vector that can produce the si-rna (plasmid or viral). for example, constructed palindromic cdna sequence introduced into cell, rna transcript into hair-pin rna, cleaved by dicer to form si- It makes up of long chains polysaccharides of mainly galacturonic acid. It"s 35% of cell wall , the rest is cellulose, hemicellulose and protein.
