JOUR 3440 Lecture Notes - Lecture 17: Thymidine, Dna-Binding Domain, Indel

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G418: neomycin, fiau, ganciclovir (gancyclovir, hsv-tk, zfn-zinc finger nucleases, zinc finger nucleases, talen, transcription activator-like effector nucleases, crispr. An antibiotic blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells. Resistance to g418 is conferred by the neo gene. Resistance to neomycin is conferred by the neo-r, neomycin resistance, gene. Hsv-tk gene, but is not toxic to cells with only the cellular tk gene. An antiviral drug kills cells containing a functional hsv-tk gene, but is not toxic to cells with only the cellular tk gene. Cells containing this gene will be killed by ganciclovir. They are a class of engineered dna-binding proteins. They facilitate targeted editing of the genome by creating double-strand breaks in dna at user-specified locations. Fusion of nonspecific dna cleavage domain from the fokl restriction endonuclease with zinc-finger proteins. The binding specificity of the designed zinc-finger domain directs the zfn to a specific genomic site.

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