JOUR 3440 Lecture Notes - Lecture 18: Base Pair, Integrase, Ribosome
24 Oct 2015
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Document Summary
Plant 1 has a disrupter gene with a block between the gene and its pdp promoter. Once crossing of both parents, recombinase is present, excision of block activates disrupter and results in sterile seed: gurt, tps, geneguard, cre. Recombinase: cre, cre-lox recombination, loxp sequence, floxed, terminator genes, rip, pdp. Genetic use restriction technology, terminator technology. make gmo plants produce non- viable seeds. Terminator technology. make gmo plants produce non-viable seeds. A tyrosine recombinase enzyme derived from the p1 bacteriophage. The enzyme is used to carry out site specific recombination events. It recombines a pair of short target sequences called the lox sequences. Locus of x-ing over p1. derived from bacteriophage p1. It means sandwiching of a dna sequence between two lox p sites. Recombination between loxp sites is catalysed by cre recombinase.
