CHMI-2227EL Lecture Notes - Lecture 14: Glutamine Synthetase, Epimerase And Racemase, Sucrase
27 Jun 2018
School
Course
Professor
Biochemistry - Day 14 2018.03.07
Questions
-Chaperones are proteins which prevent incorrect folding of proteins as well as preventing
some proteins from aggregating
-TRUE
-Cooperative binding of oxygen by hemoglobin is induced by hemoglobin
-Is induced by oxygenation
-Oxygen binding changes the 2-D conformation of hemoglobin. The consequence of this slight
change in conformation is an increase in the affinity of the other hemoglobin subunits for
Oxygen
-TRUE
-The basic structure of an antibody is made of 2 non-identical heavy polypeptide chains and 2
non-identical light polypeptide chains
-FALSE
Immunoglobulins
-The N-terminal domains of immunoglobulins are called
variable domains
-The variable domains at the end of heavy and light
chains interact with the antigen
-Variations in amino acid sequences in the variable
regions are responsible for the specificity of antibodies
within each class
-Papain (a proteolytic enzyme) found in papaya, is a
digestion produces 3 fragments called
-2 identical FAB (Antigen-binding capacity)
-1 Fc (crystallizable fragment)
-Pespsin digestion produces F(ab)2 fragments
Enzymes
-Chemical transformation in biological organisms are catalyzed by enzyme (from greek,
meaning “in yeast”
-An enzyme is a globular protein molecule which acts as a catalyst in biochemical reactions,
with the exception of some ribonucleic acids (RDAs) that catalyze their own splicing
-The RNA catalysts are known ribosomes
-A catalyst: is a compound that lowers the rate of a chemical reaction without itself being
permanently changed into another substance or consumed in the process
-Substrates: highly specific reactants for enzymes
-Enzymes can accelerate the rates of reactions as much as 1016 per uncatalyzed rates
-Ureasnis a good example:
-Catalyzed rate: 3x104 /sec
-Uncatalyzed rate: 2x10-10 /sec
-Enzymes function by lowering the energy of activation
-It takes less energy to raise the reactions to level of a activated
complex in the presence of an enzyme than in its absence
find more resources at oneclass.com
find more resources at oneclass.com
Document Summary
Chaperones are proteins which prevent incorrect folding of proteins as well as preventing some proteins from aggregating. Cooperative binding of oxygen by hemoglobin is induced by hemoglobin. Oxygen binding changes the 2-d conformation of hemoglobin. The consequence of this slight change in conformation is an increase in the af nity of the other hemoglobin subunits for. The basic structure of an antibody is made of 2 non-identical heavy polypeptide chains and 2 non-identical light polypeptide chains. The n-terminal domains of immunoglobulins are called variable domains. The variable domains at the end of heavy and light chains interact with the antigen. Variations in amino acid sequences in the variable regions are responsible for the speci city of antibodies within each class. Papain (a proteolytic enzyme) found in papaya, is a digestion produces 3 fragments called. Chemical transformation in biological organisms are catalyzed by enzyme (from greek, meaning in yeast .
