BIOL-205 Lecture Notes - Lecture 3: Influenza Vaccine, Molecular Cloning, Recombinant Dna

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Readings: chapter 7: (focus on p 147-151, 154-156, alison: chapter 8 (focus p 190-201, 215-216) Review materials: end-of-chapter questions: 1, 2b, 5 (pp 190-191, alison: chapter 8 analytical questions 1-4, 6 (pages 225-226) Dna produced by joining together in vitro genes from different sources, or which has in some way been modi ed in vitro to introduce novel genetic information. Hemagglutinin and neuraminidase are proteins on the surface of the influenza viral particle. Different versions these proteins change the viral strain (h1n1 vs h3n2) How most flu vaccine is produced: who chooses strains, viruses modified to grow in eggs, viruses propagated in chicken eggs, viruses broken open and component proteins isolated, quality testing, vaccine packaging. Issues: takes 6+ months (circulating strain can change making vaccine less effective, requires 1 egg/dose. Advantages: faster (3 months, not limited by number of eggs/size of facility (can make more) http://www. cdc. gov/flu/protect/vaccine/qa_flublok-vaccine. htm. Cut asymmetrically to leave sticky ends or symmetrically leaving blunt ends.

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