BIOL 112 Lecture Notes - Lecture 3: Protein Folding, Titration Curve, Equivalence Point

• School: McGill University
• Department: Biology
• Course: BIOL 112
• Professor: Frieder Schoeck

Document Summary

Until the solution arrives to the equivalence point, the p h rises very slowly. Then it rises fast because it just depends on the amount of naoh. At low ph(begging of titration), the acid is almost completely dissociated. At the half equivalence point, half of the solution is dissociated. At the equivalence point, 100% of the solution is dissociated. In our body, the p h is 7. We need buffer to keep the p h constant. Buffers make solutions resistant to p h change. If our p h would change, the protonation state of some groups would change. We need weak acids and weak bases, for our buffers. To make an efficient buffer, we need to make the p h at the half equivalence point. And we do this by adding 50% of acetic acid and 50% of the conjugate base. The buffer needs to go in both directions (we add the same amount of acid and its conjugate base).