BIOL 200 Lecture Notes - Lecture 10: Glutamine, Cooperative Binding, Corepressor
3 Oct 2016
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Document Summary
Plasmid 2 has lacz reporter gene and gal4 uas. The first plasmid expresses gal4 protein and get transported to plasmid 2 to express the. Full length wildtype has expression of both uas and b-gal. Taking 50 of the n- (left) terminus results in no expression. Taking the c- (right) terminus results in decreasing in expression: reduction in b-gal activity. Removing it has no transcription: activation domain. No transcription of b-gal: binding to uas require emsa. There are many dna bind and activating domains aswell as flexible protein domains. Domain swapping experiments also prove the modular nature of these proteins. If a dna-binding site fro ma one transcriptional activator is fused with the activation domain of another, a functional protein results. Recruits chromatin compacting complex and compresses so no transcription. Control regions in eukaryotes contain binding sites for multiple transcriptional activators and repressors: ex, gr-1 control region has wt1 repressors, srf/tcf activator and ap1 activator.
