BIOL 200 Lecture Notes - Lecture 17: Heterochromatin Protein 1, Dna-Binding Domain, Noncoding Dna
13 Oct 2016
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Add antibody against a particular acetylated histone tail sequence; bound nucleosomes are immunoprecipitated (centrifugation, interaction with antibody antigen) Separate dna from proteins, then determine dna sequences bound to the histones (pcr) Mono-ubiquitination: lysine, add 1 small peptide to end of a tail. More than one modification can occur on the same residue. Net result of such chromatin remodeling is to facilitate binding of transcription factors to specific. Dna sequences in chromatin; reversible process, as when proteins dissociate, dna takes back its normal configureation around the histones. Many activation domains bind to chromatin-remodeling complexes, and this binding stimulates in vitro transcription from chromatin templates (dna bound to nucleosomes) Chromatin-remodeling complexes also represses transcription by binding to transcription repression domains of repressors & fold chromatin into condensed structures. Lecture 20: the genome and general gene structure. Bacteria: compact genome, operons; genes produce polycistronic rna (one promoter, one rna, produces several polypeptides)
