BIOL 200 Lecture Notes - Lecture 6: Sister Chromatids, Mlh1, Lyase
9 Nov 2016
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Pcna acts as a clamp and ensures that the dna polymerase does(cid:374)"t fall off its su(cid:271)strate. Both strands use rna primers to initiate dna replication. Large t antigen acts as the replication fork which unwinds the eukaryotic dna. Pcna increases dna pol processivity so that once it is bound, it keeps going. Diff dna pol for lagging strand but same factors. Dna pol recognizes errored base pairing, and will back up a little bit and by backing up it switches the dna template to another domain which will chew up the errored portion and restart replication. Dna pol alpha that is used initially during anneali(cid:374)g of r(cid:374)a pri(cid:373)er, does(cid:374)"t ha(cid:448)e (cid:373)u(cid:272)h proof readi(cid:374)g ability whereas the delta and epsilon do. Cytosine to uracil by deamination (replace nh2 with double bond 0 of nh2)---change in dna backbone diff nucleotide sequence. Methylation of cytosine affects gene expression and regulation. 5 methyl cytosine---thymine through deamination now we have t=g bonded instead of c-g.
