BIOL 200 Lecture Notes - Lecture 8: Peptide, Transposable Element, Intron
23 Jan 2018
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4 different types: ltr-retrotransposon, lines, sines, processed pseudogenes. All retrotransposons also go through a process of reverse transcription: dna is coded from rna: needs reverse-transcriptase, which is a rna-dependent dna polymerase. Vs alpha- beta- delta- and epsilon- dna polymerase which is dna dependent. Transcription event: ltrs on both sides of the integrated dna (direct repeats, codes from r to poly(a) site (note: poly(a) means that there is a sequence such as aaaaaaaaaa there) Reverse transposase: yellow and red: rna, blue and green: dna, reverse transcriptase requires a primer. Ltr-retrotransposons: ltr on both ends, related to retroviruses: they are retroviruses who miss the env (envelope) region, do usually possess the group-specific antigen (gag region to reconstruct caspid) and polymerase-coding region (pol region polyprotein) Integrase: transfers similarly to dna transposition, copying works the same way as for retroviruses, mobility of ltr-retrotransposons requires transcription, experiment: Start with ty ltr-retrotransposon found in yeast.
