BIOL 200 Lecture Notes - Lecture 22: Carboy, Transcription Factor Ii H, Protein Kinase
Document Summary
Repeat: ysptsps: numbered 1-7, during transcription initiation the protein kinase associated with tfiih will phosphorylate position/searing 5. Triggers/associated with transition from initiation to elongation. The the polymerase will leave the promoter and goes slowly through proximal promoter region -> promoter clearance: another protein kinase phosphorylates the 2nd searing. Critical for ef cient elongation and promoter clearance so the polymerase engages into elongation phase. 2 phosphorylation events are critical for ef cient transcription. Phosphorylation of the ctd of large subunit of. Rna pol ii: 1st event: mediated by tfiih, 2nd event: mediated by p-tefb, end up with carboy terminal domain that is heavily phosphorylated on the repeats (most on searing 5 or 2) pre-mrna modi cations. Upon the initiation of transcription the 5" end of the emerging pre-mrna is modi ed. Ctd is critical for: capping, splicing, polyadenlyation, export (out of nucleus and into cytoplasm) If ctd is removed from yeast, organism cannot live.
