BIOL 200 Lecture Notes - Lecture 27: Nonsense-Mediated Decay, Dna-Binding Domain, Steroid Hormone Receptor
Document Summary
Lecture 27 - protein folding & quality control. Mrnas that are actively translated are more stable (protected: have a host of proteins on the 5" and 3" ends -> ribonuclease are impeded. Maturation, export and the pioneering round of translation - quality control: sr proteins de ne the exons so introns can be appropriately excised, polyadenylation of the pre-mrna, export factors are loaded onto the mrna. Pioneering round of translation = rst pass through a given mrna that has to be translated: all remaining factors bound to mrna must be removed in the pioneering round of translation - if not . Gives rise to a mutant phenotype typical of having no steroid hormone response (because not activating the steroid responsive genes) Nmd (nonsense mediated decay: mechanism that recognizes mrnas that still have factors on them and degrades them, important for getting rid of the majority of mrnas with in-frame stops.
