BIOL 200 Lecture Notes - Lecture 10: Chromosome, Non-Coding Rna, Pseudogene
Document Summary
Automated dna sequencer: dideoxy chain-termination method of dna sequencing. Dna sequence reaction mix (rxn 1): (cid:120) dna polymerase (cid:120) oligonucleotide primer (cid:120) dna template (cid:120) dntps (100 mm) (cid:120) ddatp (1 mm) (cid:198) lower concentration by 2 magnitudes. Rxns 2, 3 and 4 same as above except that ddatp is replaced by ddgtp, ddttp and ddctp, respectively. Once ddntp is incorporated into the growing dna chain, it is terminated cannot add more ddntps or dntps. Ofte(cid:374) called (cid:862)chai(cid:374) ter(cid:373)i(cid:374)ator(cid:863) (cid:120) added ddgtp in low concentration (cid:120) either dgtp or ddgtp could be incorporated (cid:120) 28 (cid:120) use gel electrophoresis to separate different-lengthed strands. Gel electrophoresis (cid:120) dna migrates from positive electrode to the negative electrode (cid:120) small fragments will move easily through gel while the big size moves slower (cid:120) a dye is used to monitor movement of dna strand. Lecture 8: the mist: the genome, eukaryotic gene structure and noncoding dna.
