BIOL 200 Lecture Notes - Lecture 28: Homeosis, Thymidine Kinase, Green Fluorescent Protein
Document Summary
Gel filtration or size exclusion chromatography separates according to size (cid:120) can separate polypeptides by column chromatography (cid:120) matrix contains pores or indents of a specific size (agarose, sephadex) If you run a buffer over that column and increase the ionic strength (usually nacl or kcl), you increase the concentration of ions. Affinity chromatography (cid:120) the most efficient method of purifying proteins (cid:120) an affinity tag can be added to engineer a translational fusion variant of any protein of interest. This facilitates rapid purification based on affinity between molecules. Transcription in eukaryotes is restricted to three organelles: (cid:120) plastids (plants, algae: mechanistically resembles prokaryotic transcription (cid:120) mitochondria, mechanistically resembles prokaryotic transcription (cid:120) nucleus, the nucleus is the most important site for transcription in eukaryotes. Tata sequence directs transcription at the promoters of some protein coding genes (cid:120) If you have a purified fraction and you put it in an enzymatic reaction, it will just initiate transcription.
