BIOL 201 Lecture 20: Secretion Vesicle Transport
Document Summary
Main means of plasma membrane getting bigger = fusing of the vesicles with the membrane (no cell can survive w/o a secretion pathway --> membrane would stay the same size, not good!) Secretion examples: neurotransmitters into a synapse; insulin (a peptide hormone) secretion; baker"s yeast secreting plasma membrane; . Main organelle involved in secretion = golgi apparatus. Golgi staining; golgi has vesicles coming and going all the time! In the bud there are secretory vesicles containing digestive enzymes which yeast secretes. Schekman let the mutagenized yeasts grow at room temperature then brought temp up to. 37 deg, where some proteins would denature and some yeasts would die. He looked at which yeasts died, and what secretory mutations those yeasts had. In d, we see a mutant that has vesicles aggregating on the inside, unable to. If you heat it up, a certain protein would denature, then vesicles can"t fuse, you can"t bud.
