BIOL 202 Lecture Notes - Lecture 9: Neurospora, Citrulline, Ornithine

• School: McGill University
• Department: Biology (Sci)
• Course: BIOL 202
• Professor: Daniel Schoen

Document Summary

Note: there was an error in the chi squared example on the slides phenotypes and gene interactions, part 2. Lecture 9: complementation testing (testing for allelism, wrap up types of interactions among loci penetrance and expressivity. Recall: mutations = tools to understand biological functions (scalpels) easiest way to go on a mutant hunt is to use a haploid organism (ie: neurospora, even though it doesnt have a lot of visible phenotypes) Beedle and tatum used x rays (ionizing radiation can cause mutations) to speed up metabolism mutations in neurospora. Test the nutritional mutants on supplemented minimal media (ex: minimal media+ amino acids) Figured that the amino acid they were lacking was r (arg) Arginine has 2 intermediates, ornithine and citrulline see chart in slides for the pattern. Complementation testing when working with diploids, need to work wt mutations that are recessive and need true breeding lines.