BIOL 202 Lecture Notes - Lecture 18: Chromosome, Ploidy, Centromere
25 Feb 2013
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It is now possible to detect huntington"s disease genes. A probe of small length, around 21 bp. Raising the temperature by a bit will not pry off the probe. A single mismatch of the probe would result in the probe falling off. Aso: allele-specific oligonucleotides, they are short enough to allow differential hybridization, but long enough to ensure locus specificity. Human genome is about 3 * 109 bp long, having 21 exact match ups in a line would be very rare. Specific probes to specific sequences would allow for tagging of genes. Then wash away the membrane to see if hybridization has taken place. This removes the need to run a gel and can be automated, but requires a prior knowledge of the sequence. The cystic fibrosis gene is extended across 250,000 bp and is organized into 24 exons and encode the. Cftr protein which forms the ion channels of epithelial cells.
