BIOL 300 Lecture Notes - Lecture 20: Retardation Factor, Cell Culture, Intron

• School: McGill University
• Department: Biology (Sci)
• Course: BIOL 300
• Professor: Siegfried Hekimi

Document Summary

Without it, the final protein would be truncated and have no function. In the wild-type yeast with this artificial gene transfected into it, the intron will be spliced out, creating mrna which can be exported into the cytoplasm. However, there is no functional beta- galactosidase produced because the protein is truncated (it"s missing the intron) and therefore the colonies don"t turn blue when treated. Another option for the wild-type is that the intron is not spliced out, and therefore the intron is retained. Shock: the mrna could be correct, but there still would not be any beta-galactosidase produced because the mrna would be stuck in the nucleus and it would never be translated. The necessary factors which identified through random mutagenesis, in which a chemical mutagen was introduced to induce mutation into the yeast genome. Another mechanism of mrna transport regulation occurs in the hiv virus, which is an rna virus.