BIOL 301 Lecture Notes - Lecture 2: Regulatory Sequence, Inbreeding, Stamen
23 Apr 2014
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We are interested in how plants regulate their size. To figure out which genes in plants are involved in regulating size, we performed random mutagenesis and identified a mutant (shorty). Problem is, we don"t know the gene that is mutated. Today, we will look at how to figure out which gene is mutated in the mutant. use of molecular dna markers as mapping landmarks. extra reference material on mapping with molecular markers in lab. for more info on pcr as a technique, take advantage of current. Protocols in molecular biology (ch 15 intro & 15. 1) available at. Mcgill life sciences library under ebooks current protocols online. Method of how you identify the gene affected by mutagenesis depends on how you mutagenized it. In general, there are two types of mutagens: insertional mutagens: a piece of dna that can integrate in random places in genome. The advantage is that you can use the.
