ANAT 262 Lecture Notes - Lecture 25: Fluorescent Tag, Lipid Droplet, Oil Red O

• School: McGill University
• Department: Anatomy & Cell Biology
• Course: ANAT 262
• Professor: John Presley

Document Summary

Dgat1/2 is in involved in tag synthesis (last step in synthesis of tag), found in er membranes. In contrast, cholesterol esters are synthesized by acat in er membranes. Vldl (hepatocytes, liver)=> idl => ldl (endocytosed), contains apob100. Hdl - apoa made by the liver and if binds to abc transporter will bind cholesterol, endocytosed in liver. Cytoplasmic organelles with a phospholipid monolayer and a core consisting of tag and cholesterol esters. Almost all cells are capable of producing lipid droplets. However, some specialized cells have far more than others. The cytoplasm of fat cells (adipocytes) consists of a single large lipid droplet. Hepatocytes and epithelial cells in mammary glands are two other cell types that contain numerous lipid droplets. Sizes range from 100 nm to several microns. E. g. foam cell is an example of a pathalogical condition involving lds. Ce and tag form clusters in er membrane (soluble up to 5%), these will enlarge and become lds.