ANAT 365 Lecture Notes - Lecture 29: Primary Biliary Cirrhosis, Atg5, Mitophagy
19 Jul 2016
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Phagophore formed to engulf mit fuse w/lysosome for degradation & recycling of aa/components. Autophagic pathways can be used to capture diff subsets of proteins all bring to lysosome. Try to use autophagy to force/boost presentation of antigens. Force macrophage express gb (not infection, just express) Full gb: to nuclear envelope (where should be) Cyto gb & mitochondrial gb: same cell line macrophage, but express gb in diff location. Antigen presentation macrophage present gb on mhc class i on surface. Specific cd8+ t cell hybridoma can only recognize 1 given peptide of gb on mhc i. After treatment, co-incubate macrophage & cd8+ t cell. If treatment is promoting presentation activated t cell makes b-galactosiadase. Treat w/mg132 or bafilomycin, see if diff in ability to present antigen, depending on where gb is. Full gb: mg132 decreases t-cell activation = need proteasome in gb presentation when on nuclear membrane.
