ANAT 381 Lecture Notes - Lecture 12: Intermediate Mesoderm, Situs Inversus, Insitu
18 Dec 2017
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When treated with uv, end up with ventralized blob. In order to find out what can rescue. If you take dorsal vegetal blastomeres (cells opposite to point of sperm entry) Rescue of uv effect by transplantation of a dorsal vegetal blastomere. Can transplant normal blastomeres into a uv-irradiated recipient and look at effect. which are secreting wnts and transplant them into the embryo, can rescue it. The same from the outside, but inside have different molecules. Molecules that can rescue the uv-ventralized" phenotype. Injected chordin mrna into the uv-treated tadpole embryo get a relatively normal tadpole developing. Chordin is expressed in mouse node, primitive streak, and axial mesoderm. Chordin is expressed in many other systems at different times of development. In mice: the node is the region at the anterior end of the primitive streak (forming along the posterior side of the embryo- at the anterior end is the node). Chordin/ noggin null mice lack anterior head structures.
