ANAT 565 Lecture Notes - Lecture 2: Cathepsin D, Plasmid, Cathepsin
3 Sep 2016
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Saposin: make it easier for hydrolases, pull out spingolipids & glycolipidids out of membrane for hydrolases to cut off head group. Proton pumps in membranes (eg channels for aa), membrane hydrolases, membrane protective proteins (eg lamp) Membrane proteins partly in lumen of tgn & cytosol. Cytosolic link to adaptor proteins, bind clathrin lysosomes. Direct link b/w adaptor & protein to be sorted. Soluble protein in tgn lumen, adaptors in cytosol can"t directly interact. Sorting receptors (eg m6pr cation independent) bind cargo & adaptor proteins. To lysosome: bind soluble protein at neutral ph, release protein in acidic ph (in endosome/lysosome) Major targeting receptor for soluble hydrolases to lysosomes. Glcnac phosphotransferase binds signal patch on hydrolase (early golgi, high oligosaccharides) Glcnac removed, leaving 1 phosphate on 6th position of mannose. Tagging occurs in cis-golgi, recognition by m6pr in trans golgi. Hydrolase into ccv at tgn fuse w/late endosome. Cargo off receptor, into lumen eventually fuse w/lysosome.
