ANAT 565 Lecture Notes - Lecture 3: Sumo Protein, Metastasis, Fyve Domain
3 Sep 2016
School
Department
Course
Professor
Document Summary
Signalling intermediates, not structural functions initiate signalling transduction pathways. Phospholipase c cleaves pip2 at phosphate head free ip3 (inositol released, phosphorylated at. Pip generates second messengers ip3 & dag, initiate signal transduction pathways. Gpcr activated g protein a-subunit-gtp dissociates from by a subunit activates plcs. Rtk activated transphosphorylation of tyrosine residues on cytoplasmic tails and subset of. Rtks (pdgf-4 & egf-r) some tyrosines in consensus binding sites for sh2 domain of plcy . Plcy to membrane phosphorylated by rtk activates activity, free to cleave pip2 to generate. Emptying calcium stores from er, then also influx from outside into cell. Huge diversity of functions of intracellular proteins regulated by calcium binding. Common catalytic domain (atp & substrate binding site) Ca tethers pkc to pm facilitates dag (at pm) interaction. Conventional: need both calcium & dag for full activation. Pseudosustrate domain at amino terminus (of pkc) mimics pkc substrate intramolecular interaction b/w pseudosubstrate & kinase domain kinase domain can"t be exposed.
